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KMID : 1007520150240062151
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Food Science and Biotechnology
2015 Volume.24 No. 6 p.2151 ~ p.2154
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Cloning of the LEU2 Gene from the Amylolytic Yeast Saccharomycopsis fibuligera
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Park Eun-Hee
Yeo Soo-Hwan
Kim Myoung-Dong
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Abstract
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The SfLEU2 structural gene of LEU2 encoding β-isopropylmalate dehydrogenase was cloned from the amylolytic yeast Saccharomycopsis fibuligera isolated from Nuruk, a Korean traditional starter for rice wine production. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 1,107 bp, including a stop codon, encoding a 368 amino acid residue. The deduced amino acid sequence of Leu2 in S. fibuligera was 76.4% homologous with Candida boidinii and Kluyveromyces marxianus sequences. The SfLEU2 gene complemented leu2Δ mutation in Saccharomyces cerevisiae, suggesting that the gene encodes a functional LEU2 in S. fibuligera. The GenBank Accession No. for SfLEU2 is KR820563.
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KEYWORD
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Saccharomycopsis fibuligera, β-isopropylmalate dehydrogenase, LEU2
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